英文名 | Immobilized Biotin Resin(Settled Resin) | ||
相关类别 | 亲和纯化介质 | 储存 | 冷藏(2-8℃) |
Uniprot ID | 生物素树脂 | ||
编 号 | 包装 | 库存 | 目录价(¥) | 您的价格(¥) | 数 量 |
C006598-0005 | 5 ML RESIN | 现货 |
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概述
Immobilized Biotin Resin is designed for the high affinity chromatography purifications of avidin, streptavidin and neutravidin protein. The resin consists of biotin coupled to 6% cross-linked agarose. Biotin, a 244 Da vitamin (Vitamin H) molecule, exhibits an extraordinary binding affinity for avidin (Ka=1015 M-1) and streptavidin. Biotin and avidin interaction is rapid and once the bond is established it can survive up to 3M guanidine-hydrochloride and extremes of pH. Biotin-avidin bonds can only be reversed by denaturing the avidin protein molecule with 8M guanidine-hydrochloride at pH1.5 or by boiling in SDS-PAGE sample loading buffer.
应用
For isolatin of avidin, streptavidin and neutravidin coupled molecules. Immunoprecipitations with avidin, streptavidin and neutravidin coupled antibodies.
组分
5 ml/bottle
属性
Biotin Binding Capacity | 2mg avidin/ml resin |
Bead Structure | 6% cross-linked agarose |
Spacer Arm | 9 |