概述

Concanavalin A (Con A) Agarose consists of Con A coupled to 6% agarose beads. Con A is a tetrameric metalloprotein lectin isolated from Canavalia ensiformis (jack bean). Con A is used for the purification of glycoproteins, polysaccharides and glycolipids as it binds molecules containing α‐D‐mannopyranosyl, α‐D‐glucopyranosyl and sterically related residues. Con A agarose has also been used in other application areas including purification of enzyme‐antibody conjugates, purification of IgM and separation of membrane vesicles.As stated above, Con A is a metalloprotein and to maintain its binding characteristics the presence of both Mn2+ and Ca2+ is essential. Each subunit of Con A utilizes one calcium and one manganese ion and these cations can be removed under acidic conditions abolishing the carbohydrate‐binding activity.

应用

For purification/ enrichment of glycoproteins, polysaccharides and glycolipids.Con A agarose has also been used in other application areas including purification of enzyme‐antibody conjugates, purification of IgM and separation of membrane vesicles.

组分

5 ml/bottle

属性

Ligand Density	10~16 mg Con A/ml resin
Capacity	20~50 mg thyroglobulin/ml resin
Bead structure	6% agarose
pH Stability	4‐9