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Order NO. D121259
兔抗SPARC多克隆抗体
英文名 Anti-SPARC rabbit ployclonal antibody
别名 ON
相关类别 一抗 储存 冷冻(-20℃)
宿主 Rabbit 反应种属 Human, Mouse, Rat
标记物 Unconjugate 克隆类型 rabbit polyclonal
抗原 SPARC    
   
编 号 包装 库存 目录价(¥) 您的价格(¥) 数 量
D121259-0025 25 ul 现货
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D121259-0100 100 ul 现货
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D121259-0200 200 ul 现货
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属性

Background: This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2015] (The background information is obtained from official databases and does not represent the product information.)
Applications: ELISA, WB, IHC
Name of antibody: SPARC
Immunogen: Fusion protein of human SPARC
Full name: secreted protein acidic and cysteine rich
Synonyms: ON; ONT; OI17; BM-40
SwissProt: P09486
ELISA Recommended dilution: 5000-10000
IHC positive control: Human liver cancer and Human bladder cancer
IHC Recommend dilution: 50-200
WB Predicted band size: 35 kDa
WB Positive control: C2C12, U251, A375 cell lysates
WB Recommended dilution: 1000-5000
相关文档
Sample Application

  • The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using D121259(SPARC Antibody) at dilution 1/70, on the right is treated with fusion protein. (Original magnification: ×200)


  • The image on the left is immunohistochemistry of paraffin-embedded Human bladder cancer tissue using D121259(SPARC Antibody) at dilution 1/70, on the right is treated with fusion protein. (Original magnification: ×200)


  • Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-3: C2C12, U251, A375 cell lysates, Primary antibody: D121259(SPARC Antibody) at dilution 1/1000, Secondary antibody: D110058(HRP-conjugated Goat anti rabbit IgG) at 1/5000 dilution, Exposure time: 10 seconds
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