Order NO. C600291
产品描述
概述
Chemokine (C-C motif) ligand 6 (CCL6), encoded by the gene CCL6, is a small cytokine belonging to the CC chemokine family that has only been identified in rodents. It signals primarily through the CCR1 receptor. CCL6 is chemotactic for B cells, CD4+ T cells, monocytes and NK cells and also exhibits powerful suppressive activity on colony formation by different lineages of hematopoietic progenitors. It contains the four highly conserved cysteine residues present in CC chemokines. The completed rat CCL6 sequence is 115 amino acids (a.a.) containing 21 a.a. signal peptide. It has about 78 % identity with mouse homologue (116 a.a.).
使用说明
This material is offered by Sangon Biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
属性
| Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder.
|
| Purity |
> 97 % by SDS-PAGE and HPLC analyses.
|
| Source |
Escherichia coli.
|
| Biological Activity |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CCR1 transfected murine BaF3 cells is in a concentration range of 10-100 ng/ml.
|
| Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 50 mM NaCl.
|
| Endotoxin |
Less than 1 EU/μg of rRtC10/CCL6 as determined by LAL method.
|
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 C. Further dilutions should be made in appropriate buffered solutions.
|
| AA Sequence |
GLIQDTVKED RPFNPTIIHQ GFQDSSDCCF SYASQIPCSR FIYYFPTSGG CTKPGIIFVT RKRKRVCANP SDQRVQTCIS TLKLGPRSGN SAIA
|
