概述

Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath et al (1), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals. The Copper Chelating Resin is specifically designed for the purification of proteins that associate with copper ions (2‐ 3), including 6x histidine tagged proteins. The Copper Chelating Resin has stronger binding affinity than Nickel, Cobalt and Copper resins, and therefore is a good choice for initial studies when little is known about the protein of interest.

组分

10 ml

属性

Ligand Density:	20~40μmoles Cu2+/ ml resin
Binding Capacity:	>50mg/ml resin
Bead Structure:	6% cross‐linked agarose