概述

Our Neonatal Cardiomyocyte Isolation System has been developed to provide researchers with a reliable, convenient, and consistent neonatal rat cardiomyocyte cell isolation method. By using purified rather than crude enzyme preparations, it has been possible to minimize the lot-to-lot variation. In addition, We use-tests the kits by isolating cardiomyocytes from neonatal rat hearts to assure performance, reliability and consistent yield of viable cells. ^The kit has been formulated in collabsoration with Dr. Ronal MacGregor. The method is based on that described by Toraason, et al. Toxicol, 56, 107 (1988) in which the minced tissue is incubated overnight with purified trypsin at 2-8°C. As pointed out by Toraason, this step reduces the hands-on time required to harvest cells compared to the time involved in sequential incubations in warm trypsin or collagenase. Purified collagenase rather than crude collagenase is used to maximize yield and viability.

应用

用于大鼠心肌细胞分离。