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Order NO. A001440
脱氧核糖核酸,变性,片段化
英文名 Deoxyribonucleic Acid, Denatured, Fragmented
相关类别 生化试剂 储存 冷冻(-20℃)
   
编 号 包装 库存 目录价(¥) 您的价格(¥) 数 量
A001440-0010 10 ML 预计90日内发货
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A001440-0050 5 X 10 ML 预计90日内发货
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产品描述

概述

We offers DNA purified from these sources: ^Calf thymus: Prepared and purified by a method developed at our company to have lower protein and RNA contamination than most other commercial preparations. This highly polymerized DNA is an excellent substrate for deoxyribonuclease. A sodium salt, it must be converted by adding magnesium ions to be susceptible to DNase. ^Calf thymus DNA, covalently bound to cellulose is also availabsle: ^DNA Cellulose, Double-Stranded ^DNA Cellulose, Single-Stranded ^Salmon Testes: Prepared by a modification of the method of Emanuel and Chaikoff, J. Biol. Chem., 203, 164, (1953). A minimum of 75% native nucleic acid. ^Salmon Testes DNA, Denatured & Fragmented is also availabsle. Prepared by mechanical shearing and heat denaturation. ^Escherichia coli: Isolated as described by Marmur, J. Mol. Biol., 3, 208, 1961. ^Lambda phage DNA is prepared from CsCl purified phage and is purified to an A260/A280 ≥1.8. Homogeneous in agarose gel electrophoresis. A solution in 10 mM Tris-HCl pH 8.0 with 1 mM EDTA. ^DNA fragments prepared by restriction endonuclease digestion of purified lambda DNA are availabsle. Supplied as solutions in 10 mM Tris-HCl pH 8.0 with 1 mM EDTA.

属性

Technical Notes One A260unit = 50 μg DNA.
Source Salmon Testes
Description Prepared from purified salmon testes DNA by mechanical shearing and heat denaturation to an average fragment size of 200-1000 base pairs. To reverse any renaturation occurring during storage this material should be briefly boiled and rapidly chilled before use. Recommended concentration for use is 100μg/ml. A solution at 5 mg/ml in 0.05M NaCl.
Storage Store at -20°C.
REQUIRES SPECIAL SHIPPING ICE PACK
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