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Order NO. A001293
l-脱氧核糖核酸,EcoR I片段
英文名 Deoxyribonucleic Acid, Lambda, EcoR I Fragments
相关类别 生化试剂 储存 冷冻(-20℃)
   
编 号 包装 库存 目录价(¥) 您的价格(¥) 数 量
A001293-0100 100 UG 预计90日内发货
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A001293-0500 5 X 100 UG 预计90日内发货
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产品描述

概述

We offers DNA purified from these sources: ^Calf thymus: Prepared and purified by a method developed at our company to have lower protein and RNA contamination than most other commercial preparations. This highly polymerized DNA is an excellent substrate for deoxyribonuclease. A sodium salt, it must be converted by adding magnesium ions to be susceptible to DNase. ^Calf thymus DNA, covalently bound to cellulose is also availabsle: ^DNA Cellulose, Double-Stranded ^DNA Cellulose, Single-Stranded ^Salmon Testes: Prepared by a modification of the method of Emanuel and Chaikoff, J. Biol. Chem., 203, 164, (1953). A minimum of 75% native nucleic acid. ^Salmon Testes DNA, Denatured & Fragmented is also availabsle. Prepared by mechanical shearing and heat denaturation. ^Escherichia coli: Isolated as described by Marmur, J. Mol. Biol., 3, 208, 1961. ^Lambda phage DNA is prepared from CsCl purified phage and is purified to an A260/A280 ≥1.8. Homogeneous in agarose gel electrophoresis. A solution in 10 mM Tris-HCl pH 8.0 with 1 mM EDTA. ^DNA fragments prepared by restriction endonuclease digestion of purified lambda DNA are availabsle. Supplied as solutions in 10 mM Tris-HCl pH 8.0 with 1 mM EDTA.

属性

Technical Notes One A260unit = 50 μg DNA.
Source Lambda DNA
Description DNA fragments prepared by the digestion of purified lambda DNA with the restriction endonuclease EcoR I. On agarose gel electrophoresis the mixture separates into five individual bands having the following number of base pairs: 21226, 7421, 5804, 4878, and 3530. A solution in 10mM Tris-HCl, pH 8.0, with 1mM EDTA.
Storage Store at -20°C.
REQUIRES SPECIAL SHIPPING DRY ICE
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