Order NO. A001293
产品描述
概述
We offers DNA purified from these sources: ^Calf thymus: Prepared and purified by a method developed at our company to have lower protein and RNA contamination than most other commercial preparations. This highly polymerized DNA is an excellent substrate for deoxyribonuclease. A sodium salt, it must be converted by adding magnesium ions to be susceptible to DNase. ^Calf thymus DNA, covalently bound to cellulose is also availabsle: ^DNA Cellulose, Double-Stranded ^DNA Cellulose, Single-Stranded ^Salmon Testes: Prepared by a modification of the method of Emanuel and Chaikoff, J. Biol. Chem., 203, 164, (1953). A minimum of 75% native nucleic acid. ^Salmon Testes DNA, Denatured & Fragmented is also availabsle. Prepared by mechanical shearing and heat denaturation. ^Escherichia coli: Isolated as described by Marmur, J. Mol. Biol., 3, 208, 1961. ^Lambda phage DNA is prepared from CsCl purified phage and is purified to an A260/A280 ≥1.8. Homogeneous in agarose gel electrophoresis. A solution in 10 mM Tris-HCl pH 8.0 with 1 mM EDTA. ^DNA fragments prepared by restriction endonuclease digestion of purified lambda DNA are availabsle. Supplied as solutions in 10 mM Tris-HCl pH 8.0 with 1 mM EDTA.
属性
Technical Notes |
One A260unit = 50 μg DNA.
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Source |
Lambda DNA
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Description |
DNA fragments prepared by the digestion of purified lambda DNA with the restriction endonuclease EcoR I. On agarose gel electrophoresis the mixture separates into five individual bands having the following number of base pairs: 21226, 7421, 5804, 4878, and 3530. A solution in 10mM Tris-HCl, pH 8.0, with 1mM EDTA.
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Storage |
Store at -20°C.
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REQUIRES SPECIAL SHIPPING |
DRY ICE
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