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Order NO. E607175
TUNEL 原位细胞凋亡检测试剂盒,POD
英文名 In Situ Cell Apoptosis Detection Kit, POD
相关类别 凋亡检测 储存 冷冻(-20℃)
   
编 号 包装 库存 目录价(¥) 您的价格(¥) 数 量
E607175-0050 50 TESTS 现货
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E607175-0100 100 TESTS 预计5日内发货
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产品描述

概述

Apoptosis is essential in many physiological processes. Several methods have been described to identify apoptotic cells. Endonucleolysis is considered as the key biochemical event of apoptosis, resulting in double-stranded, low molecular weight DNA fragments as well as single strand breaks in high molecular weight DNA. Those DNA strand breaks can be identified by labeling free 3’-OH termini with modified nucleotides in an enzymatic reaction. Terminal deoxynucleotidyl transferase (TdT) can catalyze DIG-dUTP to free 3’-OH DNA ends. The DIG-dUTP is first react with biotin-conjugated anti-digoxin antibody, which subsequently associate with streptavidin (SABC). Combined with DAB, apoptotic cells stain yellow analyzed under microscopy.

组份

Component A: Labeling Buffer; Component B: TdT (20X); Component C: DIG-dUTP (20X); Component D: Blocking Reagent; Component E: Biotin-conjugated anti-digoxin antibody; Component F: SABC (100X); Component G: Protein K (200X); Component H: Antibody Dilution Solution; Positive control samples

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