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Order NO. D261143
兔抗AKAP12多克隆抗体
英文名 Anti-AKAP12 rabbit polyclonal antibody
别名 SSeCKS; AKAP250
相关类别 一抗 储存 冷冻(-20℃)
宿主 Rabbit 反应种属 Human
标记物 Unconjugate 克隆类型 rabbit polyclonal
抗原 AKAP12    
   
编 号 包装 库存 目录价(¥) 您的价格(¥) 数 量
D261143-0025 25 ul 现货
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D261143-0100 100 ul 现货
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D261143-0200 200 ul 现货
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技术规格

Background: The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells. It associates with protein kinases A and C and phosphatase, and serves as a scaffold protein in signal transduction. This protein and RII PKA colocalize at the cell periphery. This protein is a cell growth-related protein. Antibodies to this protein can be produced by patients with myasthenia gravis. Alternative splicing of this gene results in two transcript variants encoding different isoforms.
Applications: ELISA, WB, IHC
Name of antibody: AKAP12
Immunogen: Synthetic peptide of human AKAP12
Full name: A kinase (PRKA) anchor protein 12
Synonyms: SSeCKS; AKAP250
SwissProt: Q02952
ELISA Recommended dilution: 1000-2000
IHC positive control: Human brain and Human ovarian cancer
IHC Recommend dilution: 50-200
WB Predicted band size: 191 kDa
WB Positive control: HT-29 cells
WB Recommended dilution: 200-1000
相关文档
Sample Application

  • The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using D261143(AKAP12 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200)


  • The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using D261143(AKAP12 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200)


  • Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: HT29 cells, Primary antibody: D261143(AKAP12 Antibody) at dilution 1/600, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 40 seconds
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