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Order NO. D224182
兔抗USP12多克隆抗体
英文名 Anti-USP12 rabbit polyclonal antibody
别名 ubiquitin specific peptidase 12; UBH1; USP12L1
相关类别 一抗 储存 冷冻(-20℃)
宿主 Rabbit 反应种属 Human, Mouse
标记物 Unconjugate 克隆类型 rabbit polyclonal
抗原 USP12    
   
编 号 包装 库存 目录价(¥) 您的价格(¥) 数 量
D224182-0025 25 ul 现货
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D224182-0100 100 ul 现货
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D224182-0200 200 ul 现货
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技术规格

Background: The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP12 (ubiquitin specific peptidase 12), also known as UBH1 or USP12L1, is a 370 amino acid protein belonging to the peptidase C19 family and the USP12/USP46 subfamily. Considered a deubiquitinating enzyme, it is suggested that USP12 has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have high activity. The gene encoding USP12 maps to human chromosome 13, which houses over 400 genes, such as BRCA2 and RB1, and comprises nearly 4% of the human genome.
Applications: ELISA, WB, IHC
Name of antibody: USP12
Immunogen: Full length fusion protein
Full name: ubiquitin specific peptidase 12
Synonyms: UBH1; USP12L1
SwissProt: O75317
ELISA Recommended dilution: 5000-10000
IHC positive control: Human tonsil;
IHC Recommend dilution: 25-100
WB Predicted band size: 43 kDa
WB Positive control: Mouse liver tissue lysate
WB Recommended dilution: 500-2000
相关文档
Sample Application

  • The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using D224182(USP12 Antibody) at dilution 1/35, on the right is treated with fusion protein. (Original magnification: ×200)


  • Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: Mouse liver tissue lysate, Primary antibody: D224182(USP12 Antibody) at dilution 1/700, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 second
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