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Order NO. D220484
英文名 Anti-RAF1 rabbit polyclonal antibody
别名 NS5; CRAF; Raf-1; c-Raf
相关类别 一抗 储存 冷冻(-20℃)
宿主 Rabbit 反应种属 Human, Mouse, Rat
标记物 Unconjugate 克隆类型 rabbit polyclonal
抗原 RAF1    
编 号 包装 库存 目录价(¥) 您的价格(¥) 数 量
D220484-0025 25 ul 现货
D220484-0100 100 ul 现货
D220484-0200 200 ul 现货


Background: This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.
Applications: ELISA, WB, IHC
Name of antibody: RAF1
Immunogen: Fusion protein of human RAF1
Full name: Raf-1 proto-oncogene, serine/threonine kinase
Synonyms: NS5; CRAF; Raf-1; c-Raf; CMD1NN
SwissProt: P04049
ELISA Recommended dilution: 5000-10000
IHC positive control: Human colon cancer
IHC Recommend dilution: 25-100
WB Predicted band size: 73 kDa
WB Positive control: Mouse muscle tissue and Hepg2 cell
WB Recommended dilution: 500-2000
Sample Application

  • The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using D220484(RAF1 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200)

  • Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-2: Mouse muscle tissue and Hepg2 cell, Primary antibody: D220484(RAF1 Antibody) at dilution 1/200 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds
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