网 站
反 馈
网 站
指 南
Order NO. D191001
小鼠抗6X His单克隆抗体
英文名 Anti-6×His Tag mouse monoclonal antibody
别名 6 His epitope tag
相关类别 标签抗体 储存 冷冻(-20℃) 避光
宿主 Mouse 反应种属 Fusion proteins containing the His Tag
克隆类型 mouse monoclonal 抗原 6xHis Tag
   
编 号 包装 库存 目录价(¥) 您的价格(¥) 数 量
D191001-0025 25 ul 现货
加入购物车
D191001-0100 100 ul 现货
加入购物车
D191001-0200 200 ul 现货
加入购物车
产品描述

概述

The 6 X histidine motif is used as a Tag on many recombinant proteins to facilitate purification. It can then be purified using immobilized metal ions, traditionally nickel, in a procedure termed immobilized-metal affinity chromatography. Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting. The antibody recognizes the His-Tag fused to targeted proteins in transfected or transformed cells.

应用

免疫印迹(WB),免疫荧光(IF),免疫沉淀(IP)

属性

保存缓冲液 Ascitic fluid with 0.05% sodium azide and 40% glycerol
运输条件 2-8°C
靶点全称 HHHHHH Tag
靶点别名 6 His epitope Tag
靶点研究领域 Cell Biology
亚型 IgG1
相关文档
Sample Application

  • Gel: 8%SDS-PAGE, Lysate: 2 μg, Lane: Fusion proteins containing the His Tag, Primary antibody: D191001(6×His Tag Antibody) at dilution 1/3000, Secondary antibody: Goat anti mouse IgG at 1/5000 dilution, Exposure time: 1 minute

  • The image is immunofluorescence of methanol-fixed 3T3 cells transfected with His-TAF4B vector using D191001(6×His Tag Antibody) at dilution 1/500

  • Immunoprecipitation analysis: Lane 1 is Transfected cell lysate(Input), Lane 2 is Transfected cell lysate using D191001(6×His Tag Antibody) at dilution 1/200, Lane 3 is Normal Mouse IgG(Control)
    我的购物车(0) 结算