网 站
反 馈
网 站
指 南
Order NO. D120014
兔抗PCNA多克隆抗体
英文名 Anti-PCNA rabbit polyclonal antibody
相关类别 一抗 储存 冷冻(-20℃)
宿主 Rabbit 反应种属 Human, Mouse, Rat
标记物 Unconjugate 克隆类型 rabbit polyclonal
抗原 PCNA    
   
编 号 包装 库存 目录价(¥) 您的价格(¥) 数 量
D120014-0025 25 ul 现货
加入购物车
D120014-0100 100 ul 现货
加入购物车
D120014-0200 200 ul 现货
加入购物车

属性

Background: The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. (The background information is obtained from official databases and does not represent the product information.)
Applications: ELISA, WB, IHC
Name of antibody: PCNA
Immunogen: Fusion protein of human PCNA
Full name: proliferating cell nuclear antigen
Synonyms: ATLD2
SwissProt: P12004
ELISA Recommended dilution: 1000-5000
IHC positive control: Human gasrtic cancer and Human breast cancer
IHC Recommend dilution: 50-200
WB Predicted band size: 29 kDa
WB Positive control: 293T, MCF7, Hela, RAW264.7, Jurkat cell lysates
WB Recommended dilution: 500-2000
相关文档
Sample Application

  • The image on the left is immunohistochemistry of paraffin-embedded Human gasrtic cancer tissue using D120014(PCNA Antibody) at dilution 1/44, on the right is treated with fusion protein. (Original magnification: ×200)


  • The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using D120014(PCNA Antibody) at dilution 1/44, on the right is treated with fusion protein. (Original magnification: ×200)


  • Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane 1-5: 293T, MCF7, Hela, RAW264.7, Jurkat cell lysates , Primary antibody: D120014(PCNA Antibody) at dilution 1/800, Secondary antibody: D110058(HRP-conjugated Goat anti rabbit IgG) at 1/5000 dilution, Exposure time: 30 seconds
    我的购物车(0) 结算