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Order NO. D110016
英文名 Anti-GAPDH rabbit polyclonal antibody
别名 GAPD, G3PD, MGC88685, Peptidyl-cysteine S-nitrosylase GAPDH
相关类别 一抗 储存 冷冻(-20℃)
宿主 Rabbit 反应种属 Human, Mouse, Rat
标记物 Unconjugate 克隆类型 rabbit polyclonal
抗原 GAPDH    
编 号 包装 库存 目录价(¥) 您的价格(¥) 数 量
D110016-0025 25 ul 现货
D110016-0100 100 ul 现货
D110016-0200 200 ul 现货


Background: This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants.
Applications: ELISA, WB, IHC
Name of antibody: GAPDH
Immunogen: Fusion protein of human GAPDH
Full name: glyceraldehyde-3-phosphate dehydrogenase
Synonyms: G3PD; GAPD; HEL-S-162eP
SwissProt: P04406
ELISA Recommended dilution: 5000-10000
IHC positive control: Human lung cancer
IHC Recommend dilution: 150-300
WB Predicted band size: 36 kDa
WB Positive control: 293T, Hela, NIH/3T3 cell, Mouse kidney tissue and Mouse brain tissue lysates
WB Recommended dilution: 10000-50000
Sample Application

  • The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using D110016(GAPDH Antibody) at dilution 1/110, on the right is treated with fusion protein. (Original magnification: ×200)

  • Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-5: 293T, Hela, NIH/3T3 cell, Mouse kidney tissue and Mouse brain tissue lysates, Primary antibody: D110016(GAPDH Antibody) at dilution 1/16000, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 3 minutes
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