小鼠抗E-Cadherin单克隆抗体,Anti-E-Cadherin mouse monoclonal antibody 生命科学产品与技术服务-生工生物工程(上海)股份有限公司

Order NO. C650010

小鼠抗E-Cadherin单克隆抗体

英文名	Anti-E-Cadherin mouse monoclonal antibody
相关类别	IVD抗体原料	储存	冷冻（-20℃）参照组件的存储要求

产品描述

应用

immunohistochemistry：anti-E-Cadherin mouse monoclonal antibody

1 mg/ml

Human

Background	E-Cadherin is a classical member of the cadherin superfamily. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein composed of five extracellular cadherin repeats, a transmembrane region, and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid, and ovarian cancers. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells, and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. Also, E-Cadherin has a potent invasive suppressor role and it is a ligand for integrin alpha-E/beta-7.
Product name	Anti-E-Cadherin mouse monoclonal antibody
Description	Mouse monoclonal antibody to E-Cadherin
Host species	Mouse
Tested applications	ELISA, IHC-P
Reactivity	Human
Immunogen	Synthetic peptide. The exact sequence is proprietary.
Clone number	10F6
Isotype	IgG2a
Purification	Protein A/G affinity column
Uniprot ID	P12830

相关文档

Sample Application

IHC image of E-Cadherin staining in a formalin fixed, paraffin embedded human lung adenocarcinoma (left) and breast cancer (right) tissue. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer for 20 minutes. The tissue section was then incubated with 1ug/ml mouse anti- E-Cadherin（C650010） antibody overnight at 4℃.HRP-conjugated goat anti-mouse IgG was used as secondary antibody. (Original magnification: 100X).
The image on the left is immunohistochemistry of paraffinembedded Human lung cancer tissue using E-Cadherin antibody（C650010）at 0.5 ug/ml, on the right is treated with combination of anti- E-Cadherin antibody（C650010）and synthetic peptide. (Original magnification: 200X).