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Order NO. C100222
1-生物素酰氨基-4-[4`-(甲基马来酰胺)环己烷甲酰胺]丁烷
英文名 Biotin BMCC
别名 (1-Biotinamido)-4-[4'-(maleimidomethyl)cyclohexanecarboxamido]hexane
相关类别 生物素标记试剂 储存 冷藏(2-8℃)
分子量 533.7 1-生物素酰氨基-4-[4`-(甲基马来酰胺)环己烷甲酰胺]丁烷
   
编 号 包装 库存 目录价(¥) 您的价格(¥) 数 量
C100222-0050 50 MG 现货
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产品描述

概述

Biotin-BMCC is a sulfhydryl-reactive biotinylation reagent with a long spacer arm. Maleimide-activated reagents are effective for protein modification of sulfhydryl groups. Maleimide groups react efficiently and specifically with free (reduced) sulfhydryls at pH 6.5-7.5 to form stable thioether bonds (Figure 1). Most proteins have cysteine residues whoseside-chain sulfur atoms typically occur in pairs as disulfide bonds. Reduction of these disulfide bonds exposes the sulfhydryl group required as a target for biotinylation with maleimide-activated reagents. Alternatively, sulfhydryl groups can be addedto molecules using various modification reagents (see subsequent Important Product Information Section).

应用

Biotin-BMCC is not directly soluble in water or aqueous buffers; however, if it is first dissolved in dimethylsulfoxide (DMSO) or other organic solvent, then it can be diluted and remain soluble in an aqueous buffer at typical reaction concentrations.

组份

50 mg/bottle

技术规格

Molecular Weight 533.68
Spacer arm 32.6
Purity >95% on TCL
Reaction pH acidic or neutral pH
Solubility ~4.5 mg/ml (8.5 mM) in DMSO (slightly less soluble in DMF)
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