英文名 | Concanavalin A (Con A) Agarose(Settled Resin) | ||
相关类别 | 亲和纯化介质 | 储存 | 冷藏(2-8℃) |
Uniprot ID | Con A琼脂糖 | ||
编 号 | 包装 | 库存 | 目录价(¥) | 您的价格(¥) | 数 量 |
C006216-0005 | 5 ML RESIN | 现货 |
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概述
Concanavalin A (Con A) Agarose consists of Con A coupled to 6% agarose beads. Con A is a tetrameric metalloprotein lectin isolated from Canavalia ensiformis (jack bean). Con A is used for the purification of glycoproteins, polysaccharides and glycolipids as it binds molecules containing α‐D‐mannopyranosyl, α‐D‐glucopyranosyl and sterically related residues. Con A agarose has also been used in other application areas including purification of enzyme‐antibody conjugates, purification of IgM and separation of membrane vesicles.As stated above, Con A is a metalloprotein and to maintain its binding characteristics the presence of both Mn2+ and Ca2+ is essential. Each subunit of Con A utilizes one calcium and one manganese ion and these cations can be removed under acidic conditions abolishing the carbohydrate‐binding activity.
应用
For purification/ enrichment of glycoproteins, polysaccharides and glycolipids.Con A agarose has also been used in other application areas including purification of enzyme‐antibody conjugates, purification of IgM and separation of membrane vesicles.
组分
5 ml/bottle
属性
Ligand Density | 10~16 mg Con A/ml resin |
Capacity | 20~50 mg thyroglobulin/ml resin |
Bead structure | 6% agarose |
pH Stability | 4‐9 |