概述

Enzyme‐Linked Immunosorbent Assay (ELISA) is one of the most sensitive and powerful techniques for the detection of proteins, chemicals, and drugs (antigens) in biological samples, including serum, blood and urine. The key to an ELISA is the interaction of a known antibody with the antigen of interest, where either antigen or the antibody is immobilized on an ELISA plate micro‐well. For the sensitive detection of antigen:antibody complex, secondary antibody labeled with horseradish peroxidase (HRP) is used. G‐Biosciences femtoELISA(TM) HRP kit is supplied with an enhanced blocking agent (NAP‐Blocker), an improved, ultra sensitive, stable colorimetric HRP enzyme substrate (tetramethyl benzidine; TMB), and femto‐TBST wash buffer. The kit components are enough for performing 1,000 reactions as per protocol.

特点

• Choice of HRP or AP colorimetric substrates;
• High sensitivity;
• Non-animal blocking agent to minimize cross reactivity and lower background ELISA kits and substrates for highly sensitive colorimetric detection of HRP or AP.

组分

femto-ELISA-HRP Substrate (TMB); NAP-Blocker (2X); femto-TBST (10X)