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Order NO. B002004
脱氧核糖核酸酶 I
英文名 Deoxyribonuclease I
级别 Activity ≥2,000 Kunitz U/mg dw 相关类别 生化试剂
储存 冷藏(2-8℃) 干燥    
   
编 号 包装 库存 目录价(¥) 您的价格(¥) 数 量
B002004-0005 5 MG 现货
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B002004-0020 20 MG 现货
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B002004-0100 100 MG 预计90日内发货
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产品描述

概述

Bovine pancreatic deoxyribonuclease is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3' position and phosphate group at the 5' position. The average chain length of a limit digest is a tetranucleotide.

技术规格

Stability When properly stored, all grades of our deoxyribonuclease are stable for 2-3 years. For long term storage in solution, this pproduct may be dissolved in 5mM acetate, 1mM calcium, pH 4.5 and stored in single use aliquots at -20°C or -70°C for up to one year. Only freeze and thaw once; thawed aliquots are stable refrigerated at least several weeks. Addition of 50% glycerol will maintain a liquid state at -20°C without affecting stability and material in 50% glycerol can be removed and returned to -20°C repeatedly.
Source Bovine Pancreas
Description Chromatographically purified. A lyophilized powder with glycine as a stabilizer.
Storage Store at 2-8°C. PROTECT FROM MOISTURE.
Activity ≥2,000 Kunitz units per mg dry weight
Unit Definition 1 Unit causes an increase in absorbance at 260 nm of 0.001 per minute per ml at 25°C when acting upon highly polymerized DNA at pH 5.0.Note: Kunitz units as reported by other suppliers can be 2 to 4 times higher than Kunitz units as measured at our labs. As measured at our labs, one Kunitz unit digests 1μg of lambda DNA in 10 minutes at 37°C in 50mM Tris, 1mM Mg2+, 1mM Ca2+, pH 7.8 in a 50μl reaction. Correlation of digestion units with Kunitz units is different for other DNA and buffer systems.
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