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Order NO. D190650
小鼠抗DsbA-Tag单克隆抗体
英文名 Anti-DsbA-Tag mouse monoclonal antibody
别名 periplasmic protein disulfide isomerase I; dsf; ECK3852; iarA; JW3832; ppfA
相关类别 标签抗体 储存 冷冻(-20℃)
宿主 Mouse 反应种属 E.coli
克隆类型 Mouse Monoclonal 抗原 DsbA-Tag
   
编 号 包装 库存 目录价(¥) 您的价格(¥) 数 量
D190650-0100 100 ul 现货
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产品描述

概述

DsbA has a role in cytochrome C synthesis. Required for disulfide bond formation in some periplasmic proteins such as PhoA or OmpA. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbA is reoxidized by DsbB. Required for pilus biogenesis. PhoP-regulated transcription is redox-sensitive, being activated when the periplasm becomes more reducing (deletion of dsbA/dsbB, treatment with dithiothreitol). MgrB acts between DsbA/DsbB and PhoP/PhoQ in this pathway.

应用

免疫印迹(WB)

属性

保存缓冲液 -20°C, buffer containing 0.1M Tris-Glycine(pH 7.4,150 mM NaCl)with 0.02% sodium azide,0.1mg/mlBSA and 50% glycerol
运输条件 2-8°C
靶点 Uniprot 登记号 P0AEG4
靶点全称 periplasmic protein disulfide isomerase I
靶点别名 dsf; ECK3852; iarA; JW3832; ppfA
靶点研究领域 Cell Biology
亚型 IgG2b
Sample Application

  • Gel: 10%SDS-PAGE, Lysate: 30 μg, Lane: DsbA with DsbA recombinant protein, Primary antibody: D190650(DsbA-Tag Antibody) at dilution 1/1000, Secondary antibody: Goat anti mouse IgG at 1/8000 dilution, Exposure time: 5 minutes
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