概述

Immobilized Streptavidin Resin is designed for the affinity chromatography purifications, assay development and immunopreciptations of proteins, antibodies and other molecules with a biotin tag. The resin consists of streptavidin coupled to 6% cross-linked agarose.

特点

• Streptavidin is a tetrameric protein and in many respects is similar to avidin except that it has no carbohydrate and has a slightly lower molecular weight of about 60kDa. The advantage of streptavidin is that the lack of carbohydrates significantly reduces the amount of non-specific binding.The solubility of streptavidin (isoelectric pH5) in aqueous buffer is much lower than avidin, but the binding to biotin is similar.

应用

Application for immunoprecipitation with biotinylated antibodies; Pull down assays with biotinylated proteins; Purification of biotinylated molecules including proteins, antibodies, DNA, carbohydrates.

组分

5 ml resin

属性

Bead Structure	6%Cross linked agarose for minimal leaching
Streptavidin Density	>1mg/ml/ml packed resin
Binding capacity	≥15-30 μg biotin/ml resin
Stability Range	pH 2~11
Preservative	50% slurry in PBS