概述

SPN? Protein Assay is a fast and efficient spin column method for protein estimation. It requires only 0.5‐10μg proteins per assay and no protein standards are needed. The assay is suitable for a wide range of protein samples including detergent solubilized membrane proteins and is compatible with common laboratory agents such as reducing sugars, thiols, chelating agents and detergents, including ≤2% SDS.

特点

• The concentration of protein is determined by comparing the optical density (OD595) data with the reference data on the supplied SPN? Tables. The protein concentration can be determined in <10 minutes. The SPN? Tables have been prepared using Bovine Serum Albumin (BSA) protein standards. For researcher’s convenience, we have included protein standard ODs for both spectrophotometer and microplate reader.

组分

SPN? Columns,Mini Collection Tubes,SPN? Assay Dye,SPN? Wash Buffer‐I,SPN? Wash Buffer‐II,SPN? Elution Buffer,SPN? Tables I & II: Lot Specific (with/without SDS)