ProductsSTANDARD DNA SEQUENCING SERVICES
With over 10 years of experience, Sangon is a leader in offering single and high-throughput DNA sequencing services. All DNA sequencing is performed in our own facility using the most state-of-the-art technologies and informatics.
Key Features:
·Incoming sample QC by agarose
·Fluorescent dye-terminator sequencing
·ABI Prism™ 3730xl DNA sequencers
·>650bp Q20 read lengths typical
·Universal primers provided at no additional charge (complete list)
·Template preparation and primer synthesis available
Services & Prices
Samples |
Quantity |
Price USD |
Additional charge |
Purified Plasmids |
1-10 samples |
$ 5.5 USD each |
No |
|
10-99 |
$ 4.8 USD each |
No |
|
100-500 |
$ 4.5 USD each |
No |
|
> 500 |
$ 4.2 USD each |
No |
Purified PCR Products |
1-10 samples |
$ 5.5 USD each |
No |
|
10-99 |
$ 4.8 USD each |
No |
|
100-500 |
$ 4.5 USD each |
No |
Bacterial Stabs or Culture |
1-10 samples |
$ 5.5 USD each |
Purification: $5.0 USD each |
|
10-99 |
$ 4.8 USD each |
Purification: $4.0 USD each |
|
100-500 |
$ 4.5 USD each |
Purification: $3.0 USD each |
|
> 500 |
$ 4.2 USD each |
Purification: $2.0 USD each |
Crude PCR Products |
1-10 samples |
$ 5.5 USD each |
Purification: $5.0 USD each |
|
10-99 |
$ 4.8 USD each |
Purification: $4.0 USD each |
|
100-500 |
$ 4.5 USD each |
Purification: $3.0 USD each |
|
> 500 |
$ 4.2 USD each |
Purification: $2.0 USD each |
Primer synthesis |
|
$ 0.20USD/base |
No |
Purified plasmid DNA: Plasmids should be purified using qualified Plasmid DNA Purification Kit. Quantity: Sample volume should be around 30-50ul in dd-water at a desired concentration of 100ng to 200ng/ul. Total 5ug -10ug of DNA is required per each sequencing reaction. TE is not recommended to dissolve DNA samples.
Bacterial: (1) LB stabs (2) 1 ml of overnight culture containing 15% of glycerol
Note: A. Low copy bacteria stabs or culture are not acceptable. Please send 10 ug of purified plasmid DNA. B. Please provide all necessary information including insert size, antibiotic resistance.
Purified PCR products: PCR products should be purified by qualified PCR Products Purification Kits. Purity: one band should appear on agarose gel. Sample volume should be around 10ul in dd-water at a desired concentration of 20ng/ul. Primers should be at 5-10uM in concentration and 20-50ul in volume.
Unpurified PCR products: Sample provided should be at least 2 - 3ug. Primers should be at 5-10uM in concentration and 20-50ul in volume.
Primers: We provide approximately 30 universal primers at no additional charge (see list as below). If primers for your sequencing are not included in the list, you will need to provide us the primer. Alternatively, Sangon could synthesize the primer for you at your cost. Concentration of primers: >5pmole/ul (20-50ul). Chain length of primer should be more than 15 bases, but no longer than 50 bases. Random primers as sequencing primers are not acceptable. DNA sequence, annealing temperature should be provided by the customer.
Results: Sequence chromatogram files will be sent to customer electronically. We also provide PDF alignment file upon request.
Turnaround: ~3-5 business days. For bulk orders, please inquire.
How to place an order:
You can now order your DNA sequencing online through Sangon website by filling the Online Order Form. Options are available for submitting from one to hundreds of samples.
After your order is approved, please send your samples to our distributor in your country.
If there is no distributor in your country, you may contact a distributor near your country or directly contact Sangon, but more than 100 samples as minimum order is required.
Universal Primers
Universal Primer Name |
Sequence |
3'AOX |
GCAAATGGCATTCTGACATCC |
BGH reverse |
TAGAAGGCACAGTCGAGG |
CMV-for |
CGCAAATGGGCGGTAGGCGTG |
DON1 (forward) |
TCGCGTTAACGCTAGCATGGATCTC |
DON2 (reverse) |
GTAACATCAGAGATTTTGAGACAC |
EGFP-C |
ATGGTCCTGCTGGAGTTC |
EGFP-N |
CGTCGCCGTCCAGCTCGACCAG |
GLprimer1 |
TGTATCTTATGGTACTGTAACTG |
GLprimer2 |
CTTTATGTTTTTGGCGTCTTCC |
M13 Forward |
GTAAAACGACGGCCAGT |
M13 Reverse |
CAGGAAACAGCTATGAC |
pBAD Forward |
ATGCCATAGCATTTTTATCC |
pBAD Reverse |
GATTTAATCTGTATCAGG |
pFastBacF |
GGATTATTCATACCGTCCCA |
pFastBacR |
CAAATGTGGTATGGCTGATT |
pGEX 3' |
CCGGGAGCTGCATGTGTCAGAGG |
pGEX 5' |
GGGCTGGCAAGCCACGTTTGGTG |
pQEPromotor |
CCCGAAAAGTGCCACCTG |
pQEReverse |
GTTCTGAGGTCATTACTGG |
pTriplEx 3' |
ACTCACTATAGGGCGAATTG |
pTriplEx 5' |
CTCGGGAAGCGCGCCATTGTGTTGGT |
RVprimer3 |
CTAGCAAAATAGGCTGTCCC |
RVprimer4 |
GACGATAGTCATGCCCCGCG |
S-Tag primer |
GAACGCCAGCACATGGACA |
SP6 |
ATTTAGGTGACACTATA |
T3 |
ATTAACCCTCACTAAAG |
T7 (short) |
AATACGACTCACTATAG |
T7 (long) |
AATACGACTCACTATAGgg |
T7 terminator |
GCTAGTTATTGCTCAGCGGT |