ProductsCUSTOM DNA
Features:
¡¤Much Higher Purity at Lowest Price (USD): Purity by HAP* >98.0% at USD $0.20base
¡¤Specialize in HAP* (High Affinity Purification) and PAGE Purification
¡¤World-Wide Services
¡¤High Capacity: 4000 primers / day
¡¤One of the Biggest and the Most Experienced Suppliers in the World
¡¤Fast Service: Synthesis is Completed within 24 or 36 Hours (for HAP* or PAGE )
¡¤All modified base oligos are purified by DDHPLC (WAVE system)
¡¤Highest Q.C. and Quality Guaranteed.
¡¤Wide Range Product Lines
High Affinity Purification (HAP) is a patented, novel purification method for custom oligos developed by Sangon. DMT-ON- Oligo in the crude oligo mixture is first selectively absorbed on a high affinity resin in HAP column while incomplete oligos pass through. Final oligo is produced by removing the protection group of DMT under mild acidic conditions. HAP method provides two major advantages, high purity superior to De-Salted method (>98%), and low cost comparable to PAGE or HPLC methods. Oligos produced by the HAP method has such high purity that they can be directly used for any downstream experiments such as PCR, DNA Sequencing, Gene Synthesis, and Mutagenesis. At present, the most economic method to produce oligos is the De-Salted method, which however, yields products poor in purity. For example, the purity of 20-mer, 40-mer and 60-mer is approximately 68%, 45% and 30% respectively. This is calculated based on the 4 steps in DNA synthesis: De-DMT, Coupling, Oxidation and Capping. The average yield of each cycle is about 98%. The purity of 20-mer is therefore (0.98)20-1 = 68%; of 40-mer = (0.98)40-1 = 45%; and of 60-mer = (0.98)60-1 = 30%. Most laboratories use De-Salted oligos despite of their inferior purity because higher quality oligos produced by alternative methods such as PAGE, HPLC, and OPC are too costly. HAP presents the perfect alternative for high purity oligos at lowest prices. In fact, price is even lower than those of De-Salted method in some cases. A purity photo by PAGE analysis is provided.
Please choose a right synthesis scale for oligo synthesis:
¡¡ |
50nmol |
200nmol |
1umole |
|||
¡¡ |
HAP |
PAGE/DHPLC |
HAP |
PAGE/HPLC |
HAP |
PAGE/HPLC |
0-10base |
2OD |
1 OD |
6OD |
4 OD |
20OD |
12 OD |
11-35base |
3OD |
2 OD |
10OD |
8 OD |
30OD |
25 OD |
36-60base |
3OD |
1 OD |
10OD |
4 OD |
30OD |
12 OD |
61-75base |
/ |
/ |
/ |
1 OD |
/ |
4 OD |
76-100base |
/ |
/ |
/ |
/ |
/ |
1 OD |
FORMULA: X nmol = VALUE OF OD X100 / NUMBER OF BASES
¡¤Regular Bases (<60 bases)
Scale |
Purification Method |
OD Unit |
Additional Fee (USD) |
Price (USD) / Base |
Purity of Oligo |
50nmol |
HAP |
3 |
No |
$0.20 |
>98% |
200nmol |
HAP |
10 |
No |
$0.60 |
>98% |
1umol |
HAP |
30 |
No |
$1.20 |
>98% |
50nmol |
PAGE* |
2 |
$6.00 |
$0.30 |
>99% |
200nmol |
PAGE* |
8 |
$6.00 |
$0.60 |
>99% |
1 umole |
PAGE* |
25 |
$12.00 |
$1.20 |
>99% |
50nmol |
DHPLC |
2 |
$12.00 |
$0.30 |
>99% |
200nmol |
DHPLC |
8 |
$12.00 |
$0.60 |
>99% |
1 mmole |
DHPLC |
25 |
$24.00 |
$1.20 |
>99% |
¡¤Regular Bases (61-90 bases)
Scale |
Purification Method |
OD Unit |
Additional Fee (USD) |
Price (USD) / Base |
Purity of Oligo |
200nmol |
PAGE |
1 |
$12.00 |
$1.20 |
>99% |
1umol |
PAGE |
5 |
$24.00 |
$1.80 |
>99% |
¡¤Regular Bases ( 91-110 bases)
Scale |
Purification Method |
OD Unit |
Additional Fee (USD) |
Price (USD) / Base |
Purity of Oligo |
1umol |
PAGE |
1 |
$24.00 |
$1.80 |
>99% |
¡¤Phosphrothiated
Scale |
Purification Method |
OD Unit |
Additional Fee (USD) |
Price (USD) / Base |
Purity of Oligo |
200nmol |
PAGE |
8 |
No |
$2.25 |
>99% |
1umol |
PAGE |
25 |
No |
$5.00 |
>99% |
10umol |
PAGE |
250 |
No |
$26.00 |
>99% |
¡¤dU or DI
Scale |
Purification Method |
OD Unit |
Additional Fee (USD) |
Price (USD) / Base |
Purity of Oligo |
50nmol |
HAP |
3 |
No |
$10.00 |
>98% |
200nmol |
HAP |
10 |
No |
$12.45 |
>98% |
1umol |
HAP |
30 |
No |
$19.00 |
>98% |
50nmol |
PAGE* |
2 |
$6.00 |
$10.00 |
>99% |
200nmol |
PAGE* |
8 |
$6.00 |
$12.45 |
>99% |
1 umole |
PAGE* |
25 |
$12.00 |
$19.00 |
>99% |
50nmol |
DHPLC |
2 |
$12.00 |
$10.00 |
>99% |
200nmol |
DHPLC |
8 |
$12.00 |
$12.45 |
>99% |
1 mmole |
DHPLC |
25 |
$24.00 |
$19.00 |
>99% |
¡¤3¡¯ or 5¡¯Biotin
Scale |
Purification Method |
OD Unit |
Additional Fee (USD) |
Price (USD) / Base |
Purity of Oligo |
50nmol |
DHPLC |
2 |
$40.00 |
$0.45 |
>99% |
200nmol |
DHPLC |
5 |
$60.00 |
$0.68 |
>99% |
1umol |
DHPLC |
15 |
$90.00 |
$1.35 |
>99% |
¡¤3¡¯or 5¡¯Amine
Scale |
Purification Method |
OD Unit |
Additional Fee (USD) |
Price (USD) / Base |
Purity of Oligo |
50nmol |
DHPLC |
2 |
$20.00 |
$0.45 |
>99% |
200nmol |
DHPLC |
5 |
$24.00 |
$0.68 |
>99% |
1umol |
DHPLC |
15 |
$36.00 |
$1.35 |
>99% |
¡¤5¡¯Florescence FAM, TET HEX
Scale |
Purification Method |
OD Unit |
Additional Fee (USD) |
Price (USD) / Base |
Purity of Oligo |
50nmol |
DHPLC |
2 |
$40.00 |
$0.45 |
>99% |
200nmol |
DHPLC |
5 |
$60.00 |
$0.68 |
>99% |
1umol |
DHPLC |
15 |
$90.00 |
$1.35 |
>99% |
¡¤3¡¯ or 5¡¯ Tamra or 3¡¯ or 5¡¯ Dig
Scale |
Purification Method |
OD Unit |
Additional Fee (USD) |
Price (USD) / Base |
Purity of Oligo |
50nmol |
DHPLC |
2 |
$80.00 |
$0.45 |
>99% |
200nmol |
DHPLC |
5 |
$120.00 |
$0.68 |
>99% |
1umol |
DHPLC |
15 |
$180.00 |
$1.35 |
>99% |
¡¤3¡¯or 5¡¯-SH or HS-SH
Scale |
Purification Method |
OD Unit |
Additional Fee (USD) |
Price (USD) / Base |
Purity of Oligo |
50nmol |
DHPLC |
2 |
$60.00 |
$0.45 |
>99% |
200nmol |
DHPLC |
5 |
$90.00 |
$0.68 |
>99% |
1umol |
DHPLC |
15 |
$120.00 |
$1.35 |
>99% |
¡¤5¡¯ Fam ----- 3¡¯ Dabcyl
Scale |
Purification Method |
OD Unit |
Additional Fee (USD) |
Price (USD) / Base |
Purity of Oligo |
50nmol |
DHPLC |
2 |
$180.00 |
$0.45 |
>99% |
200nmol |
DHPLC |
5 |
$250.00 |
$0.68 |
>99% |
1umol |
DHPLC |
15 |
$350.00 |
$1.35 |
>99% |
¡¤5¡¯ CY3 or CY5
Scale |
Purification Method |
OD Unit |
Additional Fee (USD) |
Price (USD) / Base |
Purity of Oligo |
50nmol |
DHPLC |
2 |
$180.00 |
$0.45 |
>99% |
200nmol |
DHPLC |
5 |
$250.00 |
$0.68 |
>99% |
1umol |
DHPLC |
15 |
$350.00 |
$1.35 |
>99% |
¡¤5¡¯Fam ------- 3¡¯Tamra (both ends) or 5¡¯Fam ------ 3¡¯Rox Taqman Probe (both ends)
Scale |
Purification Method |
OD Unit |
Additional Fee (USD) |
Price (USD) / Base |
Purity of Oligo |
50nmol |
DHPLC |
2 |
$180.00 |
$0.45 |
>99% |
200nmol |
DHPLC |
5 |
$250.00 |
$0.68 |
>99% |
1umol |
DHPLC |
15 |
$350.00 |
$1.35 |
>99% |
¡¤3¡¯ or 5¡¯ Phosphate
Scale |
Purification Method |
OD Unit |
Additional Fee (USD) |
Price (USD) / Base |
Purity of Oligo |
50nmol |
DHPLC |
2 |
$24.00 |
$0.45 |
>99% |
200nmol |
DHPLC |
5 |
$36.00 |
$0.68 |
>99% |
1umol |
DHPLC |
15 |
$60.00 |
$1.35 |
>99% |
Shipping Charge via FedEx, UPS, DHL or TNT
Area |
Shipping Charge |
Delivery Time |
Hong Kong |
$8.00 |
1-2 days |
Taiwan |
$15.00 |
1-2 days |
East-South Asia |
$20.00 |
1-2 days |
USA & Canada |
$25.00 |
1-3 days |
Europe |
$30.00 |
2-3 days |
India |
$32.00 |
2-3 days |
Other countries |
Inquiry |
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Note:
¡¤For oligos purified by HAP and PAGE, a purity photo is provided
¡¤The oligo synthesis is completed within 16 hrs for HAP and 48 hrs for PAGE.
To place an order:
¡¤Download Oligo submission form online www.sangon.com
¡¤E-mail order to: order@sangon.com
¡¤Contact your local distributor
¡¤Please indicate scales, purification method, 5¡¯ end or 3¡¯ end
¡¤To guarantee quality, we do not carry De-Salted oligos, as the purity of De-Salted oligos is too poor.